User presentation @ luingrid.guerra with the tag # steemsten
Good afternoon friends and followers, this time I seek to make my triumphal entry into the great community of @steemstem, hoping to get here a perfect niche where to live and with scientific colleagues to realize an effective symbiosis for the progress of all, interact with you in confidence as if we shared DNA.
Greetings, my name Luingrid Guerra, I am 29 years old and I am a biologist by profession and of heart, I graduated a few years ago from the prestigious Faculty of Experimental Sciences of the University of Zulia, Maracaibo Venezuela. I also studied 2 semesters of the Microbiology Postgraduate Course in the same university, but this left it unfinished unfortunately.
I am single and mother of a beautiful girl named Jeanne Paola 4 years old.
My years of studies were very diverse, Biólogia offered me many disciplines to learn and it was my duty to go looking for my vocation.
After having studied the basic subjects of the race, among those general Biology, vascular plants (Botany I), non-vascular plants (Botany II), non-vertebrate animals (Zoology I) and vertebrate animals (Zoology II), organic chemistry, inorganic , physicochemistry, biochemistry, among other relevant.
It was time to focus.
Initially I belonged to the curricular unit of the Plant Biotechnology Laboratory, where I was an active member of the study of transgenic foods, in which a group of scientists infiltrated the usual brown and green sowings, typical of the Venezuelan countryside, we specifically sought to "plant "a peculiar experiment: a milky transgenic.
This type of experiments * in vivo * activated Lorna Haynes, a scientist who has a bitter relationship against certain traits of plant technology, which is the Coordinator of the Action Network on Alternatives to the Use of Agrochemicals. They denounced the biological experiment in the Ministry of Environment and from there the prohibition in Venezuela of the sowing of organisms modified generically. What perhaps our colleague Haynes does not suspect is that his clothes, some foods of the daily consumption of Venezuelans are transgenic and that these became everyday and is so close to all of us.
Years later and after the inconveniences presented by this type of research, I decided to change my academic discipline and become part of the Environmental Microbiological Research Unit, in which I dedicated myself to characterize and quantify the microorganism species present in Lake Maracaibo Basin, San Carlos Island and Zapara Island, as well as the study of clinical samples of interest (University Hospital of Maracaibo) of patients with different types of microbial infections (intestinal, cutaneous and blood).
When I was already studying my Special Degree Work, I went to the Food Laboratory of the Chemistry Department for guidance and from the hands of professors, in which I did physical-chemical studies in different varieties of food and / or water, in parallel microbiological studies of the same to determine microorganisms present and determine whether or not they were suitable for human consumption.
My degree studies are carried out by obtaining the essential oil of orange obtained by hydrodistillation, whose concentrations would be used by the method of diffusion susceptibility in Bauer-Kirby discs to test their antimicrobial activity against microorganisms of clinical interest, so many positive gram (Staphylococcus aureus ATCC 25923, Listeria monocytogenes ATCC 19115) and gram negative (Escherichia coli ATCC 35218, Pseudomonas aeruginosa ATCC 27853). Determining that the gram-positive strains were totally resistant to pure oil, while Gram negative strains were sensitive from 10%, so we proceeded to determine the minimum inhibitory concentration, being for E. coli 9% and for P. aeruginosa 10%, with this study it was determined that this type of essential oil can be used for the microbial inhibition of pathogenic microorganisms resistant to antibiotics.
This was a bit of the wonderful and exciting world of my university studies, indescribable the sensation of each morning upon entering the Laboratory and placing my beloved white coat, surrounding myself with instruments and my inseparable microbiological companions, delving into experiments and nourishing myself with knowledge.
Being a scientist is the dream of many.
Out of curiosity i wanted to ask what dilution base did you use for the orange essential oil? And why did you choose such strain no of E.coli and Pseudomonas? Also what conditions are you maintaining for them to grow? Did you place the discs in a sterile zone between flames or a HEPA filtered zone?No where i can see that your plates are sealed with paraffin coat .How do you keep it in sterile zone? What Biosafety Level do you maintain in your lab ?
Hi luingrid.guerra..Exceptional post seems nobody saw it yet. Upvoted you as my follower.
I'm glad you liked it. You forgot to vote
I did upvote. You can check your list of upvotes.I have a equal responsibility to all my followers and i upvote them equally.I cant be partial.