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RE: [CRISPR 101] What is CRISPR?
Great post! Resteemed. I'm looking forward to reading more ;).
Recently I have come across two really interesting articles regarding the CRISPR-Cas9 system. The first is connected with the potential immune response of the human body to Cas9 proteins. The currently used homologs are derived from Staphylococcus aureus and Streptococcus pyogenes, so it is possible that the pre-existing adaptive immune responses can occur due to the contact of human population with those bacteria.
Identification of Pre-Existing Adaptive Immunity to Cas9 Proteins in Humans
The second describes the amazing possibility to modify the epigenome by bounding the Cas9 with an additional catalytic domain.
The epigenome: the next substrate for engineering
Thank you for your comment @pavlvus !
Those are two great articles, thank you for sharing. There are countless Cas9 protein fusions that have been published in the past two years but I agree that the chromatin-remodeling or nucleosome modifiers are of particular significance since epigenetics studies still lack this kind of tools.
If you are interested in other applications, one that i am particularly excited about is the RNA editing by Cas13 mutants:
http://science.sciencemag.org/content/early/2017/10/24/science.aaq0180