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RE: [CRISPR 101] What is CRISPR?

in #science7 years ago (edited)

Thank you for your comment! I also work in the CRISPR field so i'm definitely more excited than concerned about the technology :) I'm glad that you like the it, i'll be happy to have your feedback on future publications since i don't have a lot of experience in the writing of this kind of articles.

You raised some pretty interesting points here. I am not a Cpf1 expert but i think that a very interesting (and relatively unexplored) feature of it is that it produces a staggered cut, which could lead to different repair outcomes compared to Cas9. I think that in the long term we will see these different enzymes fit into a molecular toolbox that could be adapted to specific applications (longer or shorter PAM, cutting activity...). If you are interested in accuracy, here is a promising report about the Cas9 from Nesseiria Meningititis (still not peer-reviewed thought):
https://www.biorxiv.org/content/early/2017/08/04/172650

The energy question is also very interesting, I think the fact that DNA could be cleaved simply using the free energy stocked in the molecule must have been a critical reason of why it has become the support of heredity at the early stages of evolution, but this is just speculations at that point :)